In vitro effects of date palm (Phoenix dactylifera L.) pollen on colonization of neonate mouse spermatogonial stem cells

摘要: Abstract Ethnopharmacological relevance Date palm (Phoenix dactylifera L.) pollen (DPP) is widely used as a folk remedy for male infertility treatment, and has well known medicinal effects. Aim of the study This aimed to determine in vitro effects DPP on efficiency neonate mouse spermatogonial stem cells (SSCs) proliferation. Material Methods Sertoli SSCs were isolated from 6 10-days-old testes, their identity was confirmed using immunocytochemistry against cytokeratin sertoli PLZF, Oct-4 CDH-1 SSCs. Isolated testicular cultured absence or presence 0.06, 0.25 0.62 mg/ml concentrations aqueous extract 2 weeks. The number diameter SSC colonies assessed during third, 7th, 9th 14th day culture, expression Mvh, GFRα-1 evaluated quantitative PCR at end culture period. significance data analyzed ANOVA paired samples t-test Tukey Bonferroni test post hoc tests level p≤0.05. Results Pattern assay colony formation showed that numbers increased present concentration with higher slop relative other groups (P Conclusion It seems co-culture sells low doses can increase proliferation keep stemness state, while differentiate treated cells.