作者: Philip Avner , Thomas Bruls , Isabelle Poras , Lorraine Eley , Shahinaz Gas
DOI: 10.1038/NG1001-194
关键词:
摘要: Expressed-sequence tag (EST) maps are an adjunct to sequence-based analytical methods of gene detection and localization for those species which such data available, provide anchors high-density homology orthology mapping in large-scale sequencing has yet be done1. Species radiation hybrid–based transcript have been established include human2, rat3,4,5, mouse6, dog7, cat8 zebrafish9,10. We a comprehensive first-generation–placement hybrid map the mouse consisting 5,904 mapped markers (3,993 ESTs 1,911 sequence-tagged sites (STSs)). The ESTs, often originate from small-EST clusters, enriched genes expressed during early embryogenesis probably different localized humans. confirmed by situ hybridization that even singleton usually not retained studies, may represent bona fide transcribed sequences. Our studies on chromosomes 12 14 orthologous human chromosome show power our as predictive tool