Differential gene expression in the liver of the African lungfish, Protopterus annectens, after 6 days of estivation in air.

摘要: This study aimed to identify estivation-specific gene clusters through the determination of differential expressions in liver Protopterus annectens after 6 days estivation a mucus cocoon air (normoxia) using suppression subtractive hybridization polymerase chain reaction. Our results demonstrated that normoxia led up-regulation mRNA several genes related urea synthesis, including carbamoyl phosphate synthetase (Cps), argininosuccinate and glutamine synthetase. They indicate increased despite being energy-intensive, is an important adaptive response estivation. also offer indirect support proposition synthesis this lungfish involved Cps uses as substrate. In addition, up- or down-regulation occurred P. normoxia. These were prevention clot formation, activation lectin pathway for complement activation, conservation minerals (e.g. iron copper) production hemoglobin beta. Since there ribosomal proteins translational elongation factors, could be simultaneous increases protein degradation during first (the induction phase) estivation, confirming importance reconstruction structures preparation maintenance phase