Functional Mapping and Ca2+ Regulation of Nicotinic Acetylcholine Receptor Channels in Rat Hippocampal CA1 Neurons

摘要: Diverse subtypes of nicotinic acetylcholine receptors (nAChRs), including fast-desensitizing α7-containing thought to be Ca 2+ -permeable, are expressed in the CNS, where they appear regulate cognitive processing and synaptic plasticity. To understand physiological role nAChRs regulating neuronal excitability, it is important know distribution functional along surface neurons, whether can increase [Ca ] i , and/or regulated by . We mapped on membrane rat hippocampal CA1 stratum radiatum interneurons pyramidal cells acute slices recording nAChR-mediated currents elicited local UV laser-based photolysis caged carbachol patch-clamped neurons. The application (∼7 μm patches) allowed mapping soma dendritic tree, whereas fast uncaging minimized effects desensitization us measure kinetics responses. were predominant subtype interneurons, located primarily at perisomatic sites ( indicating that these native significantly consistent with previous observations made recombinant receptors. In addition, exhibited strong desensitization, rate recovery from which was controlled Our results demonstrate strategic location regulation nAChRs, may contribute understanding their involvement