Quantitative Analysis of mRNA as a Marker for Viability of Mycobacterium tuberculosis

摘要: Numerous assays which use conserved DNA or rRNA sequences as targets for amplification have been described the diagnosis of tuberculosis. However, these techniques not applied successfully to monitoring therapeutic efficacy owing persistence amplifiable nucleic acid beyond point at smears and cultures become negative. Semiquantitative analysis has used reduce time required antimicrobial susceptibility testing Mycobacterium tuberculosis, although growth up 5 days in presence some drugs is still discriminate resistant strains. The purpose present study was determine whether quantitative M. tuberculosis mRNA could be assess bacterial viability illustrate application this technique rapid determination drug susceptibility. Levels encoding 85B protein (α-antigen), IS6110 DNA, 16S were compared parallel that treated with either no drug, 0.2 μg isoniazid per ml, 1 rifampin ml. Exposure sensitive strains 24 h reduced levels <4 <0.01%, respectively, those control without drug. In contrast, did diminish over same period. Strains demonstrated reduction they nonresponsive. Quantitative offers a potentially useful tool efficacy.