Human bone marrow- and adipose-mesenchymal stem cells secrete exosomes enriched in distinctive miRNA and tRNA species.

摘要: Administration of mesenchymal stem cells (MSCs) represents a promising treatment option for patients suffering from immunological and degenerative disorders. Accumulating evidence indicates that the healing effects MSCs are mainly related to unique paracrine properties, opening opportunities secretome-based therapies. Apart soluble factors, release functional small RNAs via extracellular vesicles (EVs) seem convey essential features MSCs. Here we set out characterize full RNAome MSC-produced exosomes. We up protocol isolating exosomes released by early passage adipose- (ASC) bone marrow-MSCs (BMSC) characterized them electron microscopy, protein analysis RNA-sequencing. developed bioinformatics pipeline define exosome-enclosed RNA species performed first complete characterization BMSCs ASCs their corresponding in biological replicates. Our revealed primary have highly similar expression profiles dominated miRNAs snoRNAs (together 64-71 %), which 150–200 present at physiological levels. In contrast, miRNA pool MSC is only 2-5 % total minor subset miRNAs. Nevertheless, do not merely reflect cellular content defined overrepresented compared cell origin. Moreover, multiple expressed precluded exosomal sorting, consistent with notion these involved repression targets. While ASC BMSC class distribution composition, observed striking differences sorting evolutionary conserved tRNA seems associated differentiation status MSCs, as Sox2, POU5F1A/B Nanog expression. demonstrate exosomes, increasingly implicated intercellular communications. tRNAs species, particular halves, preferentially specific into tissue origin stemness. These findings may help understand how impact neighboring or distant possible consequences therapeutic usage.