MicroRNA-10b promotes the migration of mouse bone marrow-derived mesenchymal stem cells and downregulates the expression of E-cadherin.
作者： FENXI ZHANG , SUHUA JING , TONGMING REN , JUNTANG LIN
关键词:
摘要: The ability of mesenchymal stem cells (MSCs) to migrate is an important determinant the efficiency MSC transplant therapy. MicroRNA-10b (miR-10b) has been positively involved in migration a number tumor lineages. To date, it remains unknown whether miR-10b affects MSCs. In current study, effect on mouse bone marrow-derived MSCs (bmMSCs) was investigated. Third-passage bmMSCs were transfected with mimic and negative control precursor miRNA using Lipofectamine™ 2000. E-cadherin expression bmMSC determined. present results showed that primary exhibit spindled or triangular morphology third‑passage typical fibroblast-like morphology, exhibiting CD90-positive CD45-negative expression. Compared transfection miRNA, markedly upregulated bmMSCs, increased their downregulated observations indicate upregulation increases ability, which may be downregulation E-cadherin.
sci-hub.se 参考文章(28)
Zeynep Tokcaer-Keskin, A. Ruchan Akar, Fatma Ayaloglu-Butun, Ece Terzioglu-Kara, Serkan Durdu, Umit Ozyurda, Mehmet Ugur, Kamil C. Akcali, Timing of induction of cardiomyocyte differentiation for in vitro cultured mesenchymal stem cells: a perspective for emergencies. Canadian Journal of Physiology and Pharmacology. ,vol. 87, pp. 143- 150 ,(2009) , 10.1139/Y08-111
Jinsoo Song, Dongkyun Kim, Eun‑Jung Jin, MicroRNA-488 suppresses cell migration through modulation of the focal adhesion activity during chondrogenic differentiation of chick limb mesenchymal cells. Cell Biology International. ,vol. 35, pp. 179- 185 ,(2011) , 10.1042/CBI20100204
R Madhyastha, H Madhyastha, Y Nakajima, S Omura, M Maruyama, MicroRNA signature in diabetic wound healing: promotive role of miR-21 in fibroblast migration International Wound Journal. ,vol. 9, pp. 355- 361 ,(2012) , 10.1111/J.1742-481X.2011.00890.X
Colin Jamora, Elaine Fuchs, Intercellular adhesion, signalling and the cytoskeleton. Nature Cell Biology. ,vol. 4, pp. 101- 108 ,(2002) , 10.1038/NCB0402-E101
Lei Chen, Xiang-Rong Chen, Run Zhang, Peng Li, Yi Liu, Ke Yan, Xiao-Dan Jiang, MicroRNA-107 inhibits glioma cell migration and invasion by modulating Notch2 expression. Journal of Neuro-oncology. ,vol. 112, pp. 59- 66 ,(2013) , 10.1007/S11060-012-1037-7
Qing-jun Li, Liang Zhou, Fan Yang, Guo-xia Wang, Hang Zheng, De-sheng Wang, Yong He, Ke-feng Dou, MicroRNA-10b promotes migration and invasion through CADM1 in human hepatocellular carcinoma cells Tumor Biology. ,vol. 33, pp. 1455- 1465 ,(2012) , 10.1007/S13277-012-0396-1
Yun Xiao, Chaohan Xu, Jinxia Guan, Yanyan Ping, Huihui Fan, Yiqun Li, Hongying Zhao, Xia Li, Discovering Dysfunction of Multiple MicroRNAs Cooperation in Disease by a Conserved MicroRNA Co-Expression Network PLoS ONE. ,vol. 7, pp. e32201- ,(2012) , 10.1371/JOURNAL.PONE.0032201
Xinyang Hu, Ling Wei, Tammi M. Taylor, Jianfeng Wei, Xin Zhou, Jian-An Wang, Shan Ping Yu, Hypoxic preconditioning enhances bone marrow mesenchymal stem cell migration via Kv2.1 channel and FAK activation. American Journal of Physiology-cell Physiology. ,vol. 301, ,(2011) , 10.1152/AJPCELL.00013.2010
Joachim Oswald, Sabine Boxberger, Birgitte Jørgensen, Silvia Feldmann, Gerhard Ehninger, Martin Bornhäuser, Carsten Werner, Mesenchymal Stem Cells Can Be Differentiated Into Endothelial Cells In Vitro STEM CELLS. ,vol. 22, pp. 377- 384 ,(2004) , 10.1634/STEMCELLS.22-3-377
Kang Yan, Jie Gao, Tongtao Yang, Qiong Ma, Xiuchun Qiu, Qingyu Fan, Baoan Ma, MicroRNA-34a Inhibits the Proliferation and Metastasis of Osteosarcoma Cells Both In Vitro and In Vivo PLoS ONE. ,vol. 7, pp. e33778- ,(2012) , 10.1371/JOURNAL.PONE.0033778