Enhancing HOTAIR/MiR-10b Drives Normal Liver Stem Cells Toward a Tendency to Malignant Transformation Through Inducing Epithelial-to-Mesenchymal Transition

摘要: Previously, other groups and our team consistently have demonstrated that the possible origination of liver cancer stem cells (LCSCs) is malignant transformation from normal (LNSCs). However, this complex multi-step process far clear due to accumulation various gene dysregulations. Because non-coding RNAs (ncRNAs) could regulate multiple genes, a family even whole chromosomes, study further investigated effect dysregulated short ncRNA microRNA-10b long HOX transcript antisense RNA (HOTAIR) between LNSCs LCSCs on phenotype reversion. To clarify role in LNSCs, we used lentivirus transduction enhance miR-10b HOTAIR expression levels previously isolated rat LNSCs. The abilities proliferation, invasiveness, tumorigenesis were observed compared before after ncRNAs enhancement. After enhanced separately, several cell (CSC)-like traits appeared these including vitro-enhanced proliferative capacity, putative LCSC markers, progressive invasive ability, vivo aggravation into taking place tissue. Furthermore, strengthened partially degraded E-cadherin which one classic markers epithelial-to-mesenchymal transition (EMT). or may drive tendency toward transformation. This uncovers mechanism by promotes through down-regulating inducing EMT.