MiR-362-3p is downregulated by promoter methylation and independently predicts shorter OS of cervical squamous cell carcinoma.
作者： Lili Song , Shikai Liu , Hairong Yao , Liang Zhang , Ying Li
DOI: 10.1016/J.BIOPHA.2019.108944
关键词:
摘要: Abstract The current study was undertaken to investigate the potential influence of methylation on miR-362-5p/3p expression and further analyzed their independent prognostic value in cervical adenocarcinoma (ADC) squamous cell carcinoma (SCC) respectively. SiHa CaSki cells were used as vitro model. In silico bioinformatic analysis conducted via combined use Cancer Genome Atlas-Cervical (TCGA-CESC), Starbase 3.0 String 10.5. Results revealed that downregulation accompanied by infection high-risk human papillomavirus (HR-HPV) decreased HR-HPV cancer tissues. Demethylation could restore expression. By performing Methylation-specific PCR (MSP) based methylated or unmethylated specific primers, we confirmed proximal promoter region both lines. Higher miR-362-3p might independently predict favorable overall survival (OS) SCC patients (HR: 0.561, 95%CI: 0.354−0.889, p = 0.014), after adjustment clinical stages, lymphovascular invasion miR-362-5p However, no observed terms OS with ADC. Via analysis, found have an entirely different regulatory network ADC SCC, which help explain distinct these two histological subtypes. summary, infer level pre-miR-362 would cancer. MiR-362-3p be a biomarker but not
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