Preparation of Sera for Nutritional Studies

摘要: Publisher Summary This chapter discusses the preparation of sera for nutritional studies. Most unadapted cells grown in culture require serum as a component medium to maintain their proliferative capacity. The most commonly used procedure removal these constituents is dialysis whole serum. levels free amino acids suggest that they contribute significantly exogenous supply acids, even at 5% level. Essentially, complete by either treatment was achieved. Uric acid, total bilirubin, and plasma-bound iodine, those bound protein, were removed approximately 50% treatment. Essentially low molecular weight compounds, example, urea nitrogen glucose, cations accomplished sera. Because it advantageous prepare large quantities one time, its storage properties are importance. protein concentration treated adjusted equivalent found While differences final cell counts reproducible, did not appear result from growth factor with increased concentrations.