DNA Ploidy and Chromosome (FISH) Pattern Analysis of Peripheral Nerve Sheath Tumors

摘要: Background and methods: 44 peripheral nerve sheath tumors (PNST) (27 schwannomas, 9 neurofibromas 8 malignant (MPNST)) were analyzed to determine DNA ploidy pattern clarify the conflicting data in literature concerning this topic (whether benign PNSTs are aneuploid or not). For further insight we 6 one atypical neurofibroma five MPNSTs by fluorescence situ hybridization (FISH) technique using centromeric chromosome probes (7, 17 18) automatic image analysis station, Metafer 4. Results: Benign schwannomas (including problematic variants as ancient, cellular, neuroblastoma like multiplex schwannomas) could be characterized euploid‐polyploidisation their 4c peak height value which was usually more than 10% of total cell number measured. These characters not found among MPNST‐s. FISH revealed confirmed that ‘normal’ euploid–polyploid cells mainly eusomic–polysomic containing two, four, eight sixteen signals for each chromosomes examined, but a small proportion aneusomy tumor (average: 2.58; range 1.33–3.44). In contrast, displayed marked (18.44%) it contained normal eusomic polysomic too. Two diploid proved clearly aneusomic with trisomy monosomy 18. Conclusions: All these suggest determination combined may very useful supplementary tool making right diagnosis (to differentiate versus variants) understand better transformation PNSTs.