State-of-the-art FISHing: automated analysis of cytogenetic aberrations in interphase nuclei.
作者： Gábor Pajor , Béla Kajtár , László Pajor , Donát Alpár
DOI: 10.1002/CYTO.A.22082
关键词:
摘要: Interphase fluorescence in situ hybridization (i-FISH) is a powerful tool for visualizing various molecular targets non-dividing cells. Manual scoring of i-FISH signals labor intensive, time-consuming, and error-prone process liable to subjective interpretation. Automated evaluation signal patterns provides the opportunity overcome these difficulties. The first report on automated analysis has been published 20 years ago since then several applications have introduced fields oncology, prenatal fertility screening. In this article, we provide an insight into including its course, brief history, clinical applications, advantages challenges. lack guidelines describing new methods hampers precise comparison performance published, thus, make proposal panel parameters essential introduce standardize reproduce previously described technologies. © 2012 International Society Advancement Cytometry
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