Eight new microsatellite loci for the critically endangered fire-bellied toad Bombina bombina and their cross-species applicability among anurans

摘要: We describe eight new microsatellite loci for the critically endangered fire-bellied toad, Bombina bombina. Seven of them are polymorphic with two to seven alleles per locus, an expected heterozygosity between 0.41 and 0.8, observed 0.27 0.7. The yield was relatively low, presumably due mildly repetitive sequence motifs in flanking regions. As typical anurans, cross-species amplification limited (here, congeners orientalis variegata). Combining these those already available provides a reasonable number population studies pedigree analysis Bombina.