Synthesis of a core trisaccharide building block for the assembly of N-glycan neoconjugates

摘要: Abstract A short and high yielding synthesis of a core trisaccharide 1 as the key building block in assembly library N -glycan neoconjugates is presented. The β- d -Manp-(1→4)- -GlcpNAc linkage was introduced by inversion C-2 position β-glucoside. glucosyl donor efficiently synthesised following recently published one-pot strategy. 2-Naphthylmethyl benzylidene-acetal protection terminal mannose permitted selective liberation main branching sites for subsequent glycosylation. C5 azido linker attached to anomeric position, which stable throughout synthesis, will allow posterior immobilisation deprotected glycans on microarray surface.