HLA-G expression is regulated by miR-365 in trophoblasts under hypoxic conditions

摘要: Abstract Introduction Hypoxia occurs in the first trimester of placental development and is implicated regulation trophoblast differentiation. Prolonged hypoxic conditions placenta are related to preeclampsia. MicroRNAs (miRNAs) noncoding, single-stranded RNAs that modulate gene expression by targeting messenger RNA. We hypothesized that, under conditions, trophoblasts may have a unique miRNA profile play critical role development. Methods Total RNA was extracted from human trophoblast, HChEpC1b, exposed normoxia (20% O2) or hypoxia (2% for 24 h, profiles were investigated using microRNA array. Several differential miRNAs selected validated real-time reverse transcription PCR. identified potential targets these silico analysis. confirmed target protein western blot analysis luciferase assays. Results The miR-365 significantly upregulated conditions. In showed targeted leukocyte antigen (HLA)-G. Both overexpression inhibited HLA-G proteins. also decreased activity reporter containing 3′-untranslated region (UTR) with predicted miR-365-binding site. Discussion non-classical HLA class-Ib molecule expressed mainly extravillous which plays key maintaining immune tolerance at maternal–fetal interface. Our results indicate 3′ UTR repress its expression. an important immunoprotection semiallogenic embryo.