Cytoplasmic genome substitution in wheat affects the nuclear-cytoplasmic cross-talk leading to transcript and metabolite alterations

摘要: Alloplasmic lines provide a unique tool to study nuclear-cytoplasmic interactions. Three alloplasmic lines, with nuclear genomes from Triticum aestivum and harboring cytoplasm Aegilops uniaristata, tauschii Hordeum chilense, were investigated by transcript metabolite profiling identify the effects of cytoplasmic substitution on signaling mechanisms. In combining wheat genome H. 540 genes significantly altered, whereas 11 28 changed in carrying Ae. uniaristata or tauschii, respectively. We identified RNA maturation-related process as one most sensitive perturbation interaction. Several key components ROS chloroplast retrograde signaling, together up-regulation scavenging system, showed that changes have direct impact cross-talk. Remarkably, chilense line down-regulated some involved determination male sterility without expressing phenotype. Metabolic comparable response central metabolism euplasmic light, while exposing larger alterations compared agreement transcriptomic data. stress-related metabolites, remarkably raffinose, altered content when exposed high amino acids, well organic acids decreased. Alterations levels transcript, related photorespiration-related metabolisms associated level metabolites. The replacement species affects cross-talk leading alterations. extent these modifications was limited cytoplasm, more evident cytoplasm. consider that, this finding might be linked phylogenetic distance genomes.