Novel LanT Associated Lantibiotic Clusters Identified by Genome Database Mining

摘要: Background Frequent use of antibiotics has led to the emergence antibiotic resistance in bacteria. Lantibiotic compounds are ribosomally synthesized antimicrobial peptides against which bacteria not able produce resistance, hence making them a good alternative antibiotics. Nisin is oldest and most widely used lantibiotic, food preservation, without having developed any significant it. Having their potential limited number, there need identify novel lantibiotics. Methodology/Findings Identification lantibiotic biosynthetic clusters from an ever increasing database bacterial genomes, can provide major lead this direction. In order achieve this, strategy was adopted by screening sequenced genomes for LanT homolog, conserved transporter specific type IB clusters. This resulted identification 54 strains containing homologs, known producers. Of these, 24 were subjected detailed bioinformatic analysis genes encoding precursor peptides, modification enzyme, immunity quorum sensing proteins. Eight two LanM determinants, similar haloduracin lichenicidin identified, along with 13 single determinant as mersacidin cluster. Besides orphan homologs also identified might be associated bacteriocins, encoded somewhere else genome. Three gene had C39 domain transporter, LanBC proteins double glycine only example such cluster that salivaricin. Conclusion This study 8 putative two-component 3 genes. Putative here hold discovery lantibiotic(s).