Regeneration of adult dorsal root axons into transplants of embryonic spinal cord.

摘要: Transplants of the embryonic rat spinal cord survive and differentiate in cords adult newborn host rats. Very little is known about extent to which these homotopic transplants can provide an environment for regeneration axons that normally terminate cord. We have used horseradish peroxidase injury filling transganglionic transport methods determine whether transected dorsal roots regenerate into fetal tissue grafted Additional were examined presence calcitonin gene-related peptide-like immunoreactivity, normal horn derived exclusively from primary afferent axons. Host animals had one side L4-5 resected replaced by a transplant E14 or E15 Adjacent sectioned juxtaposed graft. The their projections then labeled 2-9 months later. tracing diffusion demonstrated severed root regenerated grown transplants. In addition, some donor neurons extended periphery at least midthigh level as indicated retrograde labeling following application tracer sciatic nerve. Primary immunoreactive peptide among those transplants, shown this immunocytochemical method exceeded techniques. Growth indicates permits axotomized would otherwise be aborted root/spinal junction. This transplantation model should therefore prove useful studying enhancement specificity regrowth