Vanillylmandelic and Homovanillic acid: Electroanalysis at non-modified and polymer-modified carbon-based electrodes

摘要: Abstract The electrochemical behaviour of two main catecholamine metabolites serving as diagnostic tumour biomarkers, homovanillic acid (HVA) and vanillylmandelic (VMA), on anodically oxidized boron doped diamond (BDD) electrodes, non-modified glassy carbon electrodes (GCE), GCE modified by polymers, namely Nafion (Nafion/GCE) poly(neutral red) (PNR/GCE), was investigated. voltammetric responses both biomarkers are strongly dependent pH the aqueous buffer solution electrode material used. BDD exhibit more positive oxidation potentials lower current densities for analytes, depending also surface termination. Concentration dependences were measured in 0.1 mol L−1 phosphate pH 3.0, optimal supporting electrolyte differential pulse determination. Achieved detection limits 0.6, 0.9, 0.8, 1.2 μmol L−1 HVA 0.4, 1.5, 2.4, 1.1 μmol L−1 VMA at GCE, Nafion/GCE, PNR/GCE, respectively. quantification organic compounds all tested materials sufficiently low their determination urine. Verification possibility simultaneous studied mixture carried out.