PEROXIDASE ISOZYMES: A MEASURE OF MOLECULAR VARIATION IN TEN HERBACEOUS SPECIES OF DATURA

摘要: Leaf proteins of the 10 herbaceous species genus Da!ura were separated by electrophoresis in starch gels and stained to reveal peroxidases. A. total 19 different sites peroxidase bands was observed for as a whole. Developmental studies showed that number increased with age leaf during seedling growth well more mature plants. Among band one particular (AL-4) varied from 4 9. Each pattern unique, no single common all species. Based on considerations interspecific cross compatibility chromosomal rearrangements c.assical taxonomic division into three sections further subdivided give five groups. The degree phylogenetic relationship within vs. between these groups assessed patterns use statistical methods based hypergeometric distribution model. probability matching due chance less among group than groups, thus indicating closer genetic association which is agreement relationships arrived at conventional methods. 1MtULTIPLE MOLECULAR forms enzymes, termed isozymes Markert Moller (1959), are known be produced wide variety higher plants (Shannon, 1968), readily demonstrable gel electrophoresis, considered products genes segregate Mendelian manner (Schwartz, 1960; Shaw, 1964; Serov, 1968; Scandalios, 1969; Marshall Allard, Sing Brewer, 1969). They therefore useful markers molecular level. In developmental studies, changing electrophoretic interpreted evidence gene activations various ontogenetic stages sampled (Scandalios, 1965; Liu, Longo, Gupta Stebbins, These may associated physiological events differentiation maturation (Racusen Foote, 1966; Siegel Galston, 1967; Kensetter Keitt, Makinen, Bhatia Nilson, plant evolution systematics, demonstration similarities differences isozyme has been used species, e.g., Nicotiana (Hart Bhatia, Sheen, 1970; Smith et al., 1 Received publication 3 March 1971. This paper dedicated Miss Sophie Satina recognition honor her 91st year. authors express their thanks Alexandra Jahn Mr. Nicholas Combatti technical assistance Keith Thompson advice computations. Research carried out Brookhaven National Laboratory under auspices U. S. Atomic Energy Commission. 1970), Phaseolus (West Garber, 1967a), Solanum (Desborough Peloquin, 1967), Fabaceae (Thurman Triticum (Johnson Hall, 1966), Oryza (Chu, 1967). Other have aimed investigating hybrid its parental West 1967b; Buiatti, Smith, Conklin 1970). study undertaken determine whether or not occur Datura, if they could mark relationships, how might change development chosen mainly because it so cytogenetically. Speciation Datura involved changes chromosome (n = 12 species), but accumulated accompanied structural chromosomes. taxonomy ten characterized distinct morphology habit growth, reviewed most recently Avery (1959). systematic had published previously others, classified sections. distinctness each maintained geographical isolation ecological preference, limit hybridization