Tombusvirus-yeast interactions identify conserved cell-intrinsic viral restriction factors.

摘要: To combat viral infections, plants possess innate and adaptive immune pathways, such as RNA silencing, R gene recessive gene-mediated resistance mechanisms. However, it is likely that additional cell-intrinsic restriction factors (CIRF) are also involved in limiting plant virus replication. This review discusses novel CIRFs with antiviral functions, many of them RNA-binding proteins or affecting the binding activities replication proteins. The against tombusviruses have been identified yeast (Saccharomyces cerevisiae), which developed an advanced model organism. Grouping based on their known cellular functions subcellular localization reveals TBSV limited by a wide variety host functions. Yeast highest connectivity network map include well-characterized Xrn1p 5’-3’ exoribonuclease, Act1p actin protein Cse4p centromere protein. important interplay between pro-viral Hsp70 chaperone co-chaperones, possibly key roles for ribosomal ribosome-associated factors. We discuss selected CIRFs, nucleolin, ribonucleases, WW-domain proteins, single- multi-domain cyclophilins, TPR-domain co-chaperones ion pumps. These frequently target region thus interfering recruitment assembly membrane-bound replicase. Although characterized act directly TBSV, we propose function “guardians” system, subverted efficiently replicase absence co-chaperones.