Expression of growth differentiation factor 9, bone morphogenetic protein 15, and anti-Müllerian hormone in cultured mouse primary follicles.

摘要: Growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15), and anti-Mullerian hormone (AMH) play an important role in the primary to secondary follicle transition activation vivo. In organ culture of neonatal mouse ovaries, it was observed that significantly fewer follicles develop stage. The objectives this study were: (1) compare ovarian follicular populations between organ-cultured ovaries freshly isolated age-matched control ovaries; (2) quantify RNA levels Gdf9, Bmp15, Amh cultured follicles; (3) immunolocalize GDF9 AMH ovaries. Ovaries from 3-day-old (PND 3) mice were for 7 or 10 days absence presence FSH. Follicular counted 13-day-old 13) Transcripts quantified using real-time RT-PCR, expressions localized immunohistochemistry. number lower than vivo controls. Gdf9 Bmp15 mRNA expression similar as (P<0.05) after day proteins respectively detected oocytes granulosa cells (GC) beginning at primordial stages onward. BMP15 production are not different controls; hence abnormal early growth related a deficient transcription these factors.