Bacillus amyloliquefaciens-secreted cyclic dipeptide – cyclo(L-leucyl-L-prolyl) inhibits biofilm and virulence production in methicillin-resistant Staphylococcus aureus

摘要: The current study explores the inhibitory efficacy of cyclo(L-leucyl-L-prolyl) (CLP), a cyclic dipeptide from Bacillus amyloliquefaciens on biofilm and virulence production methicillin-resistant Staphylococcus aureus (MRSA). minimal concentration (MIC) maximum bactericidal (MBC) CLP against three MRSA strains were found to be 256 512 μg mL−1 respectively. at its sub-MICs (16, 32, 64 128 mL−1) exhibited phenomenal dose-dependent antibiofilm activity with inhibitions 85–87%. Confocal scanning electron microscopic examinations validated CLP. In addition, was proficient enough greatly modify surface hydrophobicity significantly reduced slime synthesis MRSA. Appreciable differences noticed in EPS constituents treated signified that possible mechanism could proceed by impeding thereby prevents assemblage associated cascade. Interestingly, displayed prominent disruption (52–54%) 48 h preformed Data vivo assays using Caenorhabditis elegans unveiled non-toxic anti-infective Down-regulation all studied genes affirmed results phenotypic assays. Thus, present exemplifies use as plausible alternative conventional antibiotics controlling infections