A novel compound from the marine bacterium Bacillus pumilus S6-15 inhibits biofilm formation in Gram-positive and Gram-negative species
作者： Chari Nithya , Muthu Gokila Devi , Shunmugiah Karutha Pandian
DOI: 10.1080/08927014.2011.586127
关键词: Biology 、 Microbiology 、 Shigella flexneri 、 Extracellular polymeric substance 、 Bacillus pumilus 、 Proteus mirabilis 、 Bacillus 、 Biofilm 、 Escherichia coli 、 Bacteria
摘要: Biofilm formation is a critical problem in nosocomial infections and the aquaculture industries biofilms show high resistance to antibiotics. The aim of present study was reveal novel anti-biofilm compound from marine bacteria against antibiotic resistant Gram-positive Gram-negative biofilms. bacterial extract (50 μg ml−1) S6-01 (Bacillus indicus = MTCC 5559) showed 80–90% biofilm inhibition Escherichia coli, Shigella flexneri, Proteus mirabilis S6-15 pumilus = MTCC 5560) 80–95% all 10 tested organisms. Furthermore, they also reduced hydrophobicity index extracellular polymeric substances (EPS) production. Structural elucidation active principle using GC-MS, 1H NMR, 13C NMR spectral data revealed it be 4-phenylbutanoic acid. This first report acid as natural product. purified (10–15 μg potential activity wide range biofilm...
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