Action of cytochalasin D on DNA synthesis in cells in culture
作者： N. A. Glushankova
DOI: 10.1007/BF00835878
关键词:
摘要: To solve the problem of effect changes in actin cytoskeleton on DNA replication during action cytochalasins, long-term incubation normal cells with cytochalasin D (CCD), which selectively destroys microfilament system but does not affect transport sugars, was investigated. Incorporation labeled thymidine into mononuclear and binuclear presence CCD after its removal by rinsing also studied separately. investigate synthesis method autoradiography /sup 3/H-thymidine used. A culture mouse fibroblasts BALB/3T3 line a secondary obtained trypsinization embryos (MEF) were On MEF 3T3 cells, gradual inhibition is observed. The results indicate that structural active can abruptly reversibly disturb passage cell through cycle.
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A. Hirano, T. Kurimura, Virally transformed cells and cytochalasin B: I. The effect of cytochalasin B on cytokinesis, karyokinesis and DNA synthesis in cells Experimental Cell Research. ,vol. 89, pp. 111- 120 ,(1974) , 10.1016/0014-4827(74)90193-1
Frank J. O'Neill, Loss of Controlled Nuclear Division in BHK21 Cells Passed in Vivo Cancer Research. ,vol. 36, pp. 2019- 2024 ,(1976)
V. DEFENDI, M. G. P. STOKER, General polyploid produced by cytochalasin B. Nature. ,vol. 242, pp. 24- 26 ,(1973) , 10.1038/NEWBIO242024A0
B. Westermark, Induction of a reversible G 1 block in human glia-like cells by cytochalasin B Experimental Cell Research. ,vol. 82, pp. 341- 350 ,(1973) , 10.1016/0014-4827(73)90351-0
S. B. CARTER, Effects of Cytochalasins on Mammalian Cells Nature. ,vol. 213, pp. 261- 264 ,(1967) , 10.1038/213261A0
R. D. Estensen, P. G. W. Plagemann, Cytochalasin B: Inhibition of Glucose and Glucosamine Transport Proceedings of the National Academy of Sciences of the United States of America. ,vol. 69, pp. 1430- 1434 ,(1972) , 10.1073/PNAS.69.6.1430