Method for Simultaneous Direct Estimation of Glucose and Xylose in Serum

作者: Jesse F Goodwin

DOI: 10.1093/CLINCHEM/16.2.85

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摘要: A direct procedure for measuring serum monosaccharides is presented. Borate intensifies the colors produced when these substances react with o -toluidine, which, in case of glucose and xylose, have absorption maxima at 480 nm 630 nm, respectively. Thus, sugars may be determined singly or simultaneously a single sample by differential spectrophotometry. An alternative technique which oxidase -toluidine are used also presented estimation xylose other pentose presence glucose. These two techniques permit measurement hexose than protein solutions containing The high degree correlation reproducibility. effects such variables as reagent concentration, reaction time, borate concentration on presented, along an evaluation bilirubin hemoglobin interference.

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