Chapter 13 Antibodies to Intermediate Filament Proteins as Probes for Multiparameter Flow Cytometry of Human Cancers

摘要: Publisher Summary Cytokeratins represent the major intermediate filament protein found in all cells of epidermal origin, which include adenocarcinomas. Vimentin, expressed by nonmuscle mesenchymal is many malignant hematopoietic (lymphomas), sarcomas, and other tumors. Antibodies to cytokeratins have increasing use flow cytometric analysis breast, bladder, endocervical, squamous cell This chapter discusses proteins as markers for multiparameter analysis. Tumor-related antigens such filaments provide a means identify relatively minor populations DNA aneuploid could significant impact on tumor-specific S phase estimates diploid In addition, these stain both tumor its normal cellular counterpart, providing an internal content control that sample. With “no wash” staining technique, samples can be processed significantly less time, with lower loss reagent co human