miR-143 suppresses epithelial-mesenchymal transition and inhibits tumor growth of breast cancer through down-regulation of ERK5.

摘要: Epithelial-mesenchymal transition (EMT) plays a pivotal role in the development of cancer invasion and metastasis. Many studies have significantly enhanced knowledge on EMT through characterization microRNAs (miRNAs) influencing signaling pathways downstream events that define molecular level. In this study, we found miR-143 suppressed EMT. Up-regulating E-cadherin-mediated cell-cell adhesion ability, reduced mesenchymal markers, decreased cell proliferation, migration, vitro. vivo, xenograft mouse model also unveiled suppressive effects tumor growth. Additionally, demonstrated up-regulating extracellular signal regulated kinase 5 (ERK5) was associated with poor prognosis breast patients. Moreover, observed an inverse correlation between ERK5 tissues. directly targeted seed sequences 3'-untranslated regions ERK5. Furthermore, revealed molecules glycogen synthase 3 beta (GSK-3β)/Snail were involved modulated by summary, our findings down-regulated its target ERK5, leading to suppression induced GSK-3β/Snail cancer. © 2015 Wiley Periodicals, Inc.