Shc products are substrates of erbB-2 kinase.

摘要: The shc genes encodes three widely expressed proteins of 46, 52 and 66 kDa. Overexpression p46shc p52shc in NIH3T3 fibroblasts induces a tumorigenic phenotype. Shc products are phosphorylated on tyrosine by the activated epidermal growth factor receptor (EGFR) become physically associated with EGFR via their SH2 domain. Thus oncoproteins may play role mitogenic signal transduction. Here we report that substrates also erbB-2 kinase form complexes product intact cells. In vitro, bacterially domain is sufficient to reconstitute high affinity Shc/erbB-2 interaction. region required for binding was narrowed down most COOH-terminal 179 residues gp185erbB-2; within this region, phosphorylation one or more autophosphorylation sites Shc/gp185erbB-2 complex formation as well optimal kinase. Thus, transduction gp185erbB-2.