A novel method of demonstrating the molecular and functional equivalence between in vitro and plant-produced double-stranded RNA.
作者： William Urquhart , Geoffrey M. Mueller , Stephanie Carleton , Zihong Song , Tim Perez
DOI: 10.1016/J.YRTPH.2015.09.004
关键词:
摘要: A biotechnology-derived corn variety, MON 87411, containing a suppression cassette that expresses an inverted repeat sequence matches the of western rootworm (WCR; Diabrotica virgifera virgifera) has been developed. The expression results in formation double-stranded RNA (dsRNA) transcript 240 bp fragment WCR Snf7 gene (DvSnf7) confers resistance to by suppressing levels DvSnf7 mRNA after root feeding. Internationally accepted guidelines for assessment genetically modified crop products have developed ensure these plants are as safe food, feed, and environmental release their non-modified counterparts (Codex, 2009). As part assessments 87411 must undergo extensive requires large quantities dsRNA was produced vitro transcription (IVT). To determine if IVT is suitable surrogate 87411-produced regulatory studies, nucleotide sequence, secondary structure, functional activity each were characterized demonstrated be comparable. This comprehensive characterization indicates equivalent it studies.
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