Validation of canine uterine and testicular arteries for the functional characterisation of receptor-mediated contraction as a replacement for laboratory animal tissues in teaching

摘要: Teaching practicals for receptor physiology/pharmacology in medical and veterinary schools have involved the use of vitro experiments using tissues from laboratory animals, which been killed isolated vascular strip or ring preparations. However, scavenged has advocated to reduce animal use. Utilising discarded routine surgical procedures, such as canine neutering, not previously investigated. Canine testicular uterine (discarded tissues) were obtained neutering procedures performed by team at a local clinic stray dogs. Rings artery dissected mounted on Mulvany-Halpern wire myograph order characterize adrenergic serotonergic receptors mediating vasoconstriction. Cumulative contractile concentration-response curves constructed alpha adrenoceptor agonists epinephrine (α1 α2 receptors), phenylephrine selective) UK14304 (α2 selective). Pre-treatment with α1-selective antagonist, prazosin, was also The response serotonin (5-HT) investigated, including 5-HT (acting both 5-HT1 5-HT2 5-carboxamidotryptamine (5-CT; α-methyl (5-HT2 A observed arteries phenylephrine, prazosin caused dose-dependent parallel rightward shift dose-response curve (pA2 values 7.97 8.39, respectively). but very little appreciable arteries. maximum responses produced significantly lower than those In artery, selective agonist, 5-HT, similar administration 5-CT failed produce either segments. These results validate tissue useful source pharmacological teaching, characterizing responses.