Identification of COP9 Signalosome Subunit Genes in Bactrocera dorsalis and Functional Analysis of csn3 in Female Fecundity.

摘要: The COP9 signalosome (CSN) is an evolutionarily conserved multi-subunit complex that plays crucial roles in regulating various biological processes plants, mammals, and the model insect Drosophila. However, it poorly studied non-model insects, whereas its role fecundity remains unclear. In this study, all nine CSN subunits were identified characterized Bactrocera dorsalis, a major invasive agricultural tephritid pest. Each subunit gene, except for csn9x1, encoded protein containing PCI/PINT or MPN domain. Phylogenetic analysis revealed individually clustered into specific branch with their counterparts from other species. All genes expressed detected developmental stages tissues. Most subunits, csn8 showed highest expression level eggs. Notably, csn3 csn5 significantly enriched mature female adults. Further of was ovary ovarian gradually increased reproductive development process. RNAi-based knockdown adults reduced number laid EcRB1 USP, which encode heterodimer receptors 20E, vitellogenin transcripts (Vg1 Vg2) suppressed fat body injected csn3dsRNA. Decreased Vg1 confirmed by means Western blots. These data indicate involved reproduction 20E signaling Vg synthesis. Overall, our study may facilitate new strategies controlling B. dorsalis since provides insights evolution patterns as well critical fecundity.