Control of Cytoplasmic Maturation Events by Cytomegalovirus Tegument Protein pp150
作者： Ritesh Tandon , Edward S. Mocarski
DOI: 10.1128/JVI.00533-08
关键词:
摘要: Cytomegalovirus replication depends upon a betaherpesvirus-conserved 150-kDa tegument phosphoprotein (pp150; encoded by UL32) that supports the final steps in virion maturation at cytoplasmic assembly compartments. Amino acid substitutions were introduced into conserved region 1 (CR1) and CR2 of pp150, affecting may interact with nucleocapsids. Two independent point mutants (N201A G207A) failed to support viral evaluations transient complementation assay or after reconstruction recombinant viruses. An compartment-like inclusion developed UL32 mutant virus-infected cells was similar wild-type cells. The cellular localization trans-Golgi marker Golgin-97 suggested differences organization compartment compared Replication-defective exhibited same phenotype as virus carrying complete deletion open reading frame these assays. Electron micrographs fibroblasts 3 5 days postinfection (ΔUL32) grown on UL32-complementing showed number morphology capsids nucleus, but associated appeared highly vesiculated contained few recognizable nucleocapsids particles. These data demonstrate principle role pp150 is retain nucleocapsid through secondary envelopment compartment.
sci-hub.se 参考文章(45)
Edward S. Jr. Mocarski, Comparative analysis of herpesvirus-common proteins Cambridge University Press. ,(2007) , 10.1017/CBO9780511545313.005
G J Rosman, A D Miller, Improved Retroviral Vectors for Gene Transfer and Expression BioTechniques. ,vol. 7, pp. 980- 990 ,(1989)
Wade Gibson, Structure and assembly of the virion. Intervirology. ,vol. 39, pp. 389- 400 ,(1996) , 10.1159/000150509
Benes L. Trus, Wade Gibson, Naiqian Cheng, Alasdair C. Steven, Capsid Structure of Simian Cytomegalovirus from Cryoelectron Microscopy: Evidence for Tegument Attachment Sites Journal of Virology. ,vol. 73, pp. 2181- 2192 ,(1999) , 10.1128/JVI.73.3.2181-2192.1999
A Arvin, None, Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis Human herpesviruses: biology, therapy, and immunoprophylaxis.. ,(2007) , 10.1017/CBO9780511545313
M Chee, S A Rudolph, B Plachter, B Barrell, G Jahn, Identification of the major capsid protein gene of human cytomegalovirus. Journal of Virology. ,vol. 63, pp. 1345- 1353 ,(1989) , 10.1128/JVI.63.3.1345-1353.1989
C Roby, W Gibson, Characterization of phosphoproteins and protein kinase activity of virions, noninfectious enveloped particles, and dense bodies of human cytomegalovirus. Journal of Virology. ,vol. 59, pp. 714- 727 ,(1986) , 10.1128/JVI.59.3.714-727.1986
Lynn C Thomason, James A Sawitzke, Xintian Li, Nina Costantino, Donald L Court, None, Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination Current protocols in molecular biology. ,vol. 106, ,(2003) , 10.1002/0471142727.MB0116S78
Mark R. Underwood, Robert J. Harvey, Sylvia C. Stanat, Mary Lou Hemphill, Teresa Miller, John C. Drach, Leroy B. Townsend, Karen K. Biron, Inhibition of Human Cytomegalovirus DNA Maturation by a Benzimidazole Ribonucleoside Is Mediated through the UL89 Gene Product Journal of Virology. ,vol. 72, pp. 717- 725 ,(1998) , 10.1128/JVI.72.1.717-725.1998
Sunwen Chou, Edward S. Mocarski, Laura Hertel, Viral and cell cycle-regulated kinases in cytomegalovirus-induced pseudomitosis and replication. PLOS Pathogens. ,vol. 3, pp. 14- 22 ,(2007) , 10.1371/JOURNAL.PPAT.0030006