Viral and cell cycle-regulated kinases in cytomegalovirus-induced pseudomitosis and replication.

摘要: A process of pseudomitosis occurs during human cytomegalovirus infection that appears similar to cellular mitosis but involves the formation multiple spindle poles, abnormal condensation, and mislocalization chromosomal DNA. The relationship this viral replication cell cycle regulation has been poorly understood. Pseudomitosis consistently peaks at late times in all strains examined overall highest frequencies (30% 35% cells) using one common laboratory strain variant (AD169varATCC). Cyclin-dependent kinase 1 (Cdk1) plays a crucial role pseudomitosis, mirroring its conventional mitosis. Dominant negative Cdk1 inhibits wild-type stimulates process; however, yields remain same regardless levels. Broad inhibition cycle−regulated kinases (Cdk1/Cdk2/Cdk5/Cdk9) with indirubin-3′-monoxime substantially decreases synergizes UL97 inhibitor, maribavir. Thus, is necessary sufficient drive whereas combination important replication.