iTRAQ protein profile analysis of developmental dynamics in soybean [Glycine max (L.) Merr.] leaves.
作者： Jun Qin , Jianan Zhang , Fengmin Wang , Jinghua Wang , Zhi Zheng
DOI: 10.1371/JOURNAL.PONE.0181910
关键词:
摘要: Zao5241 is an elite soybean [Glycine max (L.) Merr.] line and backbone parent. In this study, we employed iTRAQ to analyze the proteomes protein expression profiles of during leaf development. We identified 1,245 proteins in all experiments, which only 45 had been previously annotated. Among overlapping between three biological replicates, 598 with 2 unique peptides were reliably quantified. The datasets classified into 36 GO functional terms, photosynthesis term was most significantly enriched. A total 113 defined as being differentially expressed development; 41 found be differently two four week old leaves, 84 six respectively. Cluster analysis data revealed dynamic proteomes. Proteins annotated electron carrier activity greatly enriched peak profiles, negatively modulated along whole time course. This dataset will serve foundation for a systems biology approach understanding photosynthetic
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Gene Ontology Consortium, None, The Gene Ontology (GO) database and informatics resource Nucleic Acids Research. ,vol. 32, pp. 258D- 261 ,(2004) , 10.1093/NAR/GKH036
Darren A Natale, Uma T Shankavaram, Michael Y Galperin, Yuri I Wolf, L Aravind, Eugene V Koonin, Towards understanding the first genome sequence of a crenarchaeon by genome annotation using clusters of orthologous groups of proteins (COGs) Genome Biology. ,vol. 1, pp. 1- 19 ,(2000) , 10.1186/GB-2000-1-5-RESEARCH0009
Brian C. Searle, Scaffold: A bioinformatic tool for validating MS/MS-based proteomic studies Proteomics. ,vol. 10, pp. 1265- 1269 ,(2010) , 10.1002/PMIC.200900437
Yves Meyer, Jean Grosset, Yvette Chartier, Jean-Claude Cleyet-Marel, Preparation by two-dimensional electrophoresis of proteins for antibody production: Antibodies against proteins whose synthesis is reduced by auxin in tobacco mesophyll protoplasts Electrophoresis. ,vol. 9, pp. 704- 712 ,(1988) , 10.1002/ELPS.1150091105
Michael Fountoulakis, Application of proteomics technologies in the investigation of the brain Mass Spectrometry Reviews. ,vol. 23, pp. 231- 258 ,(2004) , 10.1002/MAS.10075
M. Tester, P. Langridge, Breeding Technologies to Increase Crop Production in a Changing World Science. ,vol. 327, pp. 818- 822 ,(2010) , 10.1126/SCIENCE.1183700
Ping Lan, Wenfeng Li, Tuan-Nan Wen, Jeng-Yuan Shiau, Yu-Ching Wu, Wendar Lin, Wolfgang Schmidt, iTRAQ Protein Profile Analysis of Arabidopsis Roots Reveals New Aspects Critical for Iron Homeostasis Plant Physiology. ,vol. 155, pp. 821- 834 ,(2011) , 10.1104/PP.110.169508
Zhen Peng, Shoupu He, Wenfang Gong, Junling Sun, Zhaoe Pan, Feifei Xu, Yanli Lu, Xiongming Du, Comprehensive analysis of differentially expressed genes and transcriptional regulation induced by salt stress in two contrasting cotton genotypes. BMC Genomics. ,vol. 15, pp. 760- 760 ,(2014) , 10.1186/1471-2164-15-760
Wells W. Wu, Guanghui Wang, Seung Joon Baek, Rong-Fong Shen, Comparative study of three proteomic quantitative methods, DIGE, cICAT, and iTRAQ, using 2D gel- or LC-MALDI TOF/TOF Journal of Proteome Research. ,vol. 5, pp. 651- 658 ,(2006) , 10.1021/PR050405O
Pinghua Li, Lalit Ponnala, Neeru Gandotra, Lin Wang, Yaqing Si, S Lori Tausta, Tesfamichael H Kebrom, Nicholas Provart, Rohan Patel, Christopher R Myers, Edwin J Reidel, Robert Turgeon, Peng Liu, Qi Sun, Timothy Nelson, Thomas P Brutnell, The developmental dynamics of the maize leaf transcriptome Nature Genetics. ,vol. 42, pp. 1060- 1067 ,(2010) , 10.1038/NG.703