Glucosamine, a naturally occurring amino monosaccharide, inhibits A549 and H446 cell proliferation by blocking G1/S transition

摘要: Uncontrolled proliferation is important in tumorigenesis. In the present study, effects of glucosamine on lung cancer cell were investigated. The expression cyclin E, one key cyclins G1/S transition, and Skp2, ubiquitin ligase subunit that targets negative cycle regulator, p27Kip1, also assessed. Moreover, underlying mechanisms action investigated cells. A549 H446 cells synchronized using thymidine presence or absence glucosamine. effect was determined by MTT assay. Cyclin E p27Kip1 proteins their phosphorylation levels detected western blot analysis. Furthermore, evaluated flow cytometry. Glucosamine found to inhibit suppress Skp2 expression. Notably, (Thr62) (Thr187) downregulated glucosamine, negatively correlated with degradation. arrest phase. Thus, may blocking transition through inhibition protein