CD4-Independent Human Immunodeficiency Virus Infection Involves Participation of Endocytosis and Cathepsin B
作者： Hiroaki Yoshii , Haruka Kamiyama , Kensuke Goto , Kazunori Oishi , Nobuhiko Katunuma
DOI: 10.1371/JOURNAL.PONE.0019352
关键词:
摘要: During a comparison of the infectivity mNDK, CD4-independent human immunodeficiency virus type 1 (HIV-1) strain, to various cell lines, we found that HeLa cells were much less susceptible than 293T and TE671 cells. Hybridoma between as cells, suggesting cellular factors enhance mNDK infection in By screening cDNA expression library cystatin C was isolated an enhancer infection. Because cathepsin B protease, natural ligand C, upregulated speculated high levels activities inhibitory enhanced by impairing excessive activity. Consistent with this idea, pretreatment 125 µM CA-074Me, inhibitor, resulted 8-fold enhancement infectivity. is activated low pH acidic endosomes, further examined potential roles endosomes Suppression endosome acidification or endocytosis inhibitors Eps15 dominant negative mutant reduced which CD4-dependent infections not significantly impaired. Taken together, these results suggest endocytosis, endosomal acidification, activity are involved entry HIV-1.
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