Homology-based annotation of non-coding RNAs in the genomes of Schistosoma mansoni and Schistosoma japonicum

摘要: Schistosomes are trematode parasites of the phylum Platyhelminthes. They considered most important human helminth in terms morbidity and mortality. Draft genome sequences now available for Schistosoma mansoni japonicum. Non-coding RNA (ncRNA) plays a crucial role gene expression regulation, cellular function defense, homeostasis, pathogenesis. The genome-wide annotation ncRNAs is non-trivial task unless well-annotated genomes closely related species already available. A homology search structured ncRNA S. resulted 23 types with conserved primary secondary structure. Among these, we identified rRNA, snRNA, SL RNA, SRP, tRNAs RNase P, also possibly MRP 7SK RNAs. In addition, confirmed five miRNAs that have recently been reported japonicum found two additional homologs known miRNAs. tRNA complement comparable to free-living planarian Schmidtea mediterranea, although some amino acids differences more than factor observed: Leu, Ser, His overrepresented, while Cys, Meth, Ile underrepresented mansoni. On other hand, number reduced by four. Both schistosomes complete set minor spliceosomal snRNAs. Several expected exist were not found, among them telomerase vault RNAs, Y structures presented here represent dataset from any lophotrochozoan so far. This data provides an reference further analysis indeed eukaryotic at large.