An Ultra-Rapid Biosensory Point-of-Care (POC) Assay for Prostate-Specific Antigen (PSA) Detection in Human Serum.

摘要: Prostate-specific antigen (PSA) is the established routine screening tool for detection of early-stage prostate cancer. Given laboratory-centric nature process, development a portable, ultra rapid high-throughput system PSA highly desirable. In this study, an advancedpoint-of-care in human serum was developed based on cellular biosensor where cell membrane modified by electroinserting specific antibody against PSA. Thirty nine samples were used validation biosensory detection. Samples analyzed parallel with standard immunoradiometric assay (IRMA) and electrochemical immunoassay comparison purposes. They classified three different concentration ranges (0, <4 ≥4 ng/mL). Cells membrane-engineered 0.25 μg/mL anti-PSA demonstrated statistically lower response upper (≥4 ng/mL) range. addition, cell-based performed better than immunosensor terms sensitivity resolution positive containing ng/mL spite its preliminary, proof-of-concept stage development, could be as aninitiative fast, low-cost, POC