Differential effect of hypoxia on etoposide‐induced DNA damage response and p53 regulation in different cell types

摘要: Among the main causes of cancer cell resistance to chemotherapy are p53 mutation and hypoxic tumor microenvironment. However, effect hypoxia can be very different from one type other. We studied on etoposide-induced death in two lines, HepG2 A549 cells. Hypoxia decreased apoptosis cells but not Here, we evidenced pathways, known play important roles resistance, that differently affected by these types. First, cells, protein level activity acting post-transcriptionally independently HIF-1. The results suggest an translation. On other hand, no was observed level. Secondly, DNA damage response while this case Indeed, a decrease phosphorylation CHK2 H2AX with ATM observed. Importantly, prevent at levels effects strongly cell-type dependent. J. Cell. Physiol. 228: 2365–2376, 2013. © 2013 Wiley Periodicals, Inc.