MicroRNA-1285 inhibits the expression of p53 by directly targeting its 3' untranslated region

摘要: The well-known tumor suppressor p53 plays critical roles in the modulation of multiple cellular processes. regulation is complicated and remains elusive. In this study, we used a high-throughput luciferase reporter screen to demonstrate that can be regulated by microRNA-1285 (miR-1285). Notably, miR-612, which has same seed sequence as miR-1285, cannot bind 3' untranslated region (3' UTR) p53. Mutational analyses confirmed UTR mRNA contains two miR-1285 target sites, are nearly perfectly complementary mature sequence. Ectopic expression inhibits protein. contrast, silencing increases expression. Furthermore, transcription p21, master gene downstream conclusion, our findings provide first evidence directly regulates targeting its UTR.