Extracellular microRNAs in bronchoalveolar lavage samples from patients with lung diseases as predictors for lung cancer

摘要: Abstract Objective The detection of tumor-associated microRNA expression in bronchial lavage (BL) samples lung cancer patients could improve the non-invasive tumor diagnostic. Methods profile extracellular microRNAs was evaluated using three pools for group (malignant) and non-cancerous (benign) 10 each. To confirm results selected a qRT-PCR BL 30 different used. We examined total-RNA from cell-free supernatant samples. For normalization we added exogenous cel-miR 39. Results Using arrays found panel eight (hsa-miR 19b-1, 1285, 1289, 1303, 217, 29a-5p, 548-3p, 650) that were differentially expressed between group. Further investigation by qPCR revealed five (U6 snRNA, hsa-miR significantly up-regulated with cancer. Conclusions In identified this study have diagnostic potential use as noninvasive biomarkers