Plasma miR-324-3p and miR-1285 as diagnostic and prognostic biomarkers for early stage lung squamous cell carcinoma

摘要: // Xujie Gao 1, 3, 4 , Yang Wang Hua Zhao Feng Wei Xinwei Zhang 2, Yanjun Su 5 Changli Hui Li Xiubao Ren 1 Department of Immunology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China 2 Biotherapy, 3 National Clinical Research Center Cancer, Key Laboratory Immunology Lung Correspondence to: Ren, email: renxiubao@tjmuch.com Li, lihui@tjmuch.com Keywords: lung squamous cell carcinoma, plasma, miRNA, diagnostic marker, prognostic marker Received: April 19, 2016      Accepted: June 30, Published: August 11, 2016 ABSTRACT Background: Specific biomarkers for early detection outcome prediction carcinoma (LSCC) are still lacking. This study assessed the differentially expressed miRNAs as potential stage LSCC. Results: Base on results multi-phase study, we found that miR-324-3p was significantly up-regulated, whereas mir-1285 down-regulated in plasma I LSCC patients compared to healthy controls. ROC analysis showed AUC miR-1285 were 0.79 0.85, respectively. The combination these two could further improve accuracy (AUC = 0.89). multivariate revealed level an independent predictor Methods: 395 195 controls enrolled this study. We screened using TaqMan Low Density Arrays (TLDA) followed by three-phase qRT-PCR validation. also evaluated association candidate with overall survival patients. Finally, target genes analyzed public available databases bioinformatics methods. Conclusions: current suggests levels serve markers while may a