Polyvalent display libraries

摘要: The invention is directed to inter alia two related but self-sufficient improvements in conventional display methods. first improvement provides methods of enriching libraries for members displaying more than one copy a polypeptide prior affinity screening such with target interest. These can achieve diverse populations which the vast majority retaining full-length coding sequences encode polypeptides having specific target. In second aspect, subcloning nucleic acids encoding displayed enriched from vector an expression without need clonal isolation individual members. result polyclonal antibodies and other use, e.g., as diagnostic or therapeutic reagents.