Cloning of the sacS gene encoding a positive regulator of the sucrose regulon in Bacillus subtilis
作者： Michel Débarbouillé , Frank Kunst , André Klier , Georges Rapoport
DOI: 10.1111/J.1574-6968.1987.TB02184.X
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摘要: Abstract The sacS gene controls the expression of 2 saccharolytic enzymes in Bacillus subtilis (sucrase and levansucrase). This paper describes a recombinant plasmid containing mutant allele, sacSc. was isolated from B. DNA bank established Escherichia coli. Moreover, it shown that sacSc placed on high-copy plasmid, is dominant over wild-type chromosomal sacS+ allele. result strongly suggests encodes positive regulatory protein.
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