Double role of pyruvate kinase type M2 in the regulation of phosphometabolite pools

摘要: Proliferating nonnal cells and tumor typically express a particular isoenzyme of pyruvate kinase called type M 2-PK. This exists in two different forms: the so-called 2-PK, which is strongly overexpressed cells, lung predominant species found lung. The 2-PK mainly appears trimeric or dimeric state has low affinity for its substrate phosphoenolpyruvate. always tetrameric high phophoenolpyruvate. Using temperature-sensitive mutant pp60v-src kinase, we have investigated influence transfonnation on amount fonns M2 host cells. appearance detectable 3 h maximal 12 after onset follows activation tyrosine kinase. level specific phosphorylation serine tyrosine, whereas can hardly be detected. From this from vitro experiments, conclude that leads to breakdown PEP-affinity fonn within correlates with an increase glycolytic phosphometabolite pools synthetic products derived them, e.g., P-ribose-PP. Some these phosphometabolites, AMP NADH, represent links between metabolism cell proliferation.