Analysis of mouse embryonic patterning and morphogenesis by forward genetics

摘要: Many aspects of the genetic control mammalian embryogenesis cannot be extrapolated from other animals. Taking a forward approach, we have induced recessive mutations by treatment mice with ethylnitrosourea and identified 43 that affect early morphogenesis patterning, including 38 genes not been studied previously. The molecular lesions responsible for 14 were identified, in nine had characterized Some vertebrate-specific components conserved signaling pathways; example, at least five previously uncharacterized regulators Sonic hedgehog (Shh) pathway. Approximately half all initial establishment body plan, several these produce phenotypes described A large fraction cell migration, cellular organization, structure. findings indicate phenotype-based screens provide direct unbiased method to identify essential development.