An in vitro study, evaluating the effect of sunitinib and/or lapatinib on two glioma cell lines.

摘要: Aim We sought to assess the effect of sunitinib and lapatinib applied either alone or in combination, on U87 M059K glioma cells. Methods Both cell lines were cultured as recommended by manufacturer. Sunitinib applied, separately cells after attachment at doses 10nM, 100nM, 1 μM 10 μM. To determine whether agents affect proliferation cells, 3-[4,5-dimethylthiazol-2-yl]-2,5 dimethyltetrazolium bromide assay was used. Apoptosis detected using annexin V/propidium iodide detection assay, migration performed 24-well microchemotaxis chambers. The release MMPs into culture medium measured zymography. Results agents, administered decreased a dose-dependent manner 48 h their application both lines. inhibition combination statistically different than each agent alone. increased inhibited combination. levels remained unaffected However, MMP-9 MMP-2 treatment with lapatinib. Conclusion and/or appear exhibit significant effects proliferation, apoptosis When alone, appears be more potent inhibitor