The immune response to p53 in breast cancer patients is directed against immunodominant epitopes unrelated to the mutational hot spot.
作者： Yann Legros , David Lane , Beata Schlichtholz , Fabien Calvo , Michel Marty
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摘要: Alteration of the p53 gene is most frequent genetic feature human cancer and leads to overexpression altered protein in tumor cell nucleus. Two diagnostic procedures are currently available assess mutations: ( a ) molecular analysis sequence; b immunohistochemical accumulation. We now report third approach, serological analysis. Fifteen % primary breast patients were found have circulating antibodies by immunoprecipitation or immunoblotting. close correlation between presence such bad prognosis as high histological grade absence hormone receptors. Furthermore, we that B-cell response induced two immunodominant regions located at carboxy amino termini protein, outside central mutational hot spot region. These findings suggest analysis, combined with histochemical methods, may be suitable for assessing state patients.
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