An in vitro investigation of endocrine disrupting effects of the mycotoxin alternariol

摘要: Abstract Alternariol (AOH) is a mycotoxin commonly produced by Alternaria alternata on wide range of foods. Few studies to date have been performed evaluate the effects AOH endocrine activity. The present study makes use in vitro mammalian cellular based assays and gene expression investigate ability act as an disruptor various modes action. Reporter (RGAs), incorporating natural steroid hormone receptors for oestrogens, androgens, progestagens glucocorticoids were used identify disruption at level nuclear receptor transcriptional activity, H295R steroidogenesis assay was assess production. exhibited weak oestrogenic response when tested oestrogen responsive RGA binding progesterone progestagen shown be synergistically increased presence AOH. cells exposed 0.1–1000 ng/ml AOH, did not cause significant change testosterone cortisol hormones but exposure 1000 ng/ml (3.87 μM) resulted increase estradiol In following only one NR0B1 down-regulated, whereas mRNA CYP1A1, MC2R, HSD3B2, CYP17, CYP21, CYP11B2 CYP19 up-regulated. Expression other genes investigated significantly. conclusion that also has interfere with pathway.